Immunoinformatics-based design of multi-epitope subunit vaccine against human metapneumovirus
Personal Biography
Ubaidullah is a passionate professional with a Bachelor's degree in Biotechnology from Shaheed Benazir Bhutto University, Sheringal, Dir Upper. With a strong interest in health and molecular sciences, Ubaidullah has gained valuable experience working in the Infectious Disease Laboratory at Quaid-e-Azam University, Islamabad. His research focuses on the innovative field of immuno-informatics, particularly the design of a multi-epitope subunit vaccine against Human Metapneumovirus (hMPV).
In addition to his laboratory experience, Ubaidullah has been active in freelance assignment writing, specializing in bioinformatics topics such as bioinformatics tools and docking. His professional journey also includes working in a medical store and participating in various international conferences and seminars.
Ubaidullah is deeply committed to promoting peace, democratic values, and social harmony through his work and volunteer activities. He was a member of the PSF Federation and Skill Society at his university, where he organized numerous events to foster a sense of community and engagement among students.
Currently, Ubaidullah is pursuing further studies and research opportunities. He has applied for MS programs in Molecular and Cellular Biology at the University of Waikato, Biotechnology at the University of Canterbury, and Plant Breeding at Lincoln University. He aims to advance his research on vaccine design for hMPV, contributing significantly to the field of molecular biology.
Outside of his academic and professional pursuits, Ubaidullah enjoys playing football and singing in Pashto and Urdu. His dedication to both his professional growth and personal interests highlights his versatile and proactive nature
Paper Abstract
ABSTRACT
Human metapneumovirus (hMPV) is a respiratory virus, first reported during 2001 in the Netherlands. The hMPV is enveloped, negative-sense, single-stranded RNA virus, and belongs to the family Pneumoviridae and genus Metapneumovirus. It is one of the leading causative agents of acute lower respiratory tract infections (ALRTIs), which is characterized by symptoms like bronchiolitis, wheezing, coughing and pneumonia in newborn, children, elderly and immunocompromised individuals. Currently, no licensed vaccines are available for the prevention of hMPV-associated disease. Here, we used immune informatics approaches and targeted the fusion and glycoprotein of the hMPV to develop multiepitope subunit vaccines (MESV) against hMPV. Various online tools predicted the best epitopes from fusion and glycoprotein of the hMPV. The predicted epitopes were joined together by different linkers and subjected to 3D structure prediction. The strong binding of the constructed vaccines with human TLR4 was verified by the molecular docking approach. The GC content and codon adaptation index (CAI) values confirmed high expression of the constructed vaccines in the pET-28a (+) expression vector. The immune response simulation data indicated that the injected vaccines robustly activate the immune system, triggering the production of high titers of IgG and IgM antibodies. In conclusion, this study provided a solid base of concept to develop dynamic and effective vaccine against hMPV. Further wet lab experiment should be carried out to demonstrate efficacy and safety of the designed MESV against the hMPV.
Key words: Human metapneumovirus, TLR4, vaccine, molecular docking
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